Journal: BioMed Research International

Article Title: Potential of Using Cell-Free DNA and miRNA in Breast Milk to Screen Early Breast Cancer

doi: 10.1155/2020/8126176

Figure Lengend Snippet: Identification of BM cfRNA deriving mainly from breast-derived cells. RNAs of BM cfRNAs and BM cellular RNA from three different donors (donors 7, 8, and 9) were subjected to RT-PCR to detect mRNA levels of (a) highly expressed genes in breast-related and (b) highly expressed genes in blood-related cells. The graphs show normalized −ΔCt values for the indicated genes (described in detail in Supplemental Text). All error bars denote SEM, n = 3. (c) The heat map of methylation levels in every identical reliable slide window of different samples. WGBS data was analyzed with default parameters (1000 bp slide windows, 500 bp overlap). For accuracy, only slide windows with more than 3000 total C reads (methylated and unmethylated) were regarded as reliable for comparison between samples. The graph was drawn with Log2 methylated percentages after mean subtraction and standardization and arranged in descending order across all chromosomes of the gDNA6 sample. (d) The chromosome-separated heat map of methylation levels in identical reliable slide windows of different samples. The graph was drawn with Log2 methylated percentages after mean subtraction and standardization and arranged in descending order in each chromosome of the gDNA6 sample.

Article Snippet: miRNA sequencing and whole genome bisulfite sequencing (WGBS) were performed by LC Sciences (Houston, TX, USA).

Techniques: Derivative Assay, Reverse Transcription Polymerase Chain Reaction, Methylation, Comparison

Journal: Frontiers in Immunology

Article Title: A Systematic Review of Tissue and Single Cell Transcriptome/Proteome Studies of the Brain in Multiple Sclerosis

doi: 10.3389/fimmu.2022.761225

Figure Lengend Snippet: An overview of the advantages and disadvantages of the omics techniques.

Article Snippet: Epigenomics , Molecular changes on the DNA , Assessment of variability of factors that regulate the genome without changing the DNA sequence , WGBS (whole genome bisulfite-treated DNA sequencing) , Methylome-wide pattern and alterations , Moderate , Complex data analysis, lack of functional knowledge on methylation at other sites , Whole methylome state on single base pair level.

Techniques: Sequencing, Genome Wide, DNA Sequencing, Functional Assay, Methylation, Microarray, Hybridization, Immunoprecipitation, DNA Binding Assay, Binding Assay, Gene Expression, Amplification, Expressing, Clone Assay, Multiplexing, Protein-Protein interactions, Blocking Assay, Microscopy, Activity Assay, Mass Spectrometry, Mass Cytometry